The Critical Role of Surface Chemistry in Protein Microarrays . ROLE OF TARGET IDENTIFICATION AND TARGET VALIDATION IN DRUG DISCOVERY Presented by:- Pallavi Duggal M.Pharmacy (2nd Semester) Department of Pharmacology ISF … Microarrays are widely used to address a plethora of scientific questions in the pharmaceutical industry, particularly in drug discovery and development. PROTEIN MICROARRAYS Protein microarray is an emerging technology that provides a versatile platform for characterization of hundreds of thousands of proteins in a highly parallel and high-throughput way. The PMT amplifies the signal from each photon of light, which is converted into a digital value used to create the image representing signal intensity at each pixel position. Drug discovery Applicable in pharmacogenomics Comparative analysis of gene Help the identification of specific proteins produce by diseased cells. DNA microarrays are … This presentation will take you through the concept of microarray and its application in detail. GPCRs are successfully used as drug targets because the binding of natural ligands to their paired GPCR(s) can be moderated by appropriate small molecule drugs 1., 2..Effective engineering of these drugs is essential, however, because aberrant binding to such a physiologically significant target class can lead to serious side effects. http://www.bio.davidson.edu/courses/genomics/chip/chip.html. Protein microarrays allow for the simultaneous and rapid analysis of thousands of proteins in high-throughput (HT). ... 1.3.2.2 DnA microarrays Applications in Drug Discovery and Development Identifying the drug target The majority of available drugs have protein molecules as their targets. Zhu Photobleaching is the destruction of a fluorophore by high intensity light, resulting in reduced signal intensity and consequent underestimation of expression level. Drug metabolism in pharmaceutical research has traditionally focused on the well-defined aspects of absorption, distribution, metabolism and excretion, commonly-referred to ADME properties of a compound, particularly in the areas of metabolite identification, identification of drug metabolizing enzymes (DMEs) and associated metabolic pathways, and reaction mechanisms. Pre-hybridisation of microarrays is a valuable step for reducing non specific hybridisation. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. 3. 1467-1476. Protein microarrays Martyna Franczuk Functional protein microarrays differ from analytical arrays in that functional protein arrays are made of full-length functional ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 56be2a-YmNmY Any DNA in the sample that is complementary to a probe sequence will become bound to the chip. Chappell, and S.H. There are many different robots on the market; this is a Genetix spotting robot located at the Mouse Genetics Unit in Harwell, Oxfordshire. Clipping is a handy way to collect important slides you want to go back to later. Role of Target Identification and Target Validation in Drug Discovery Process 1. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Microarray technology uses chips with attached DNA sequences as probes for gene expression. See our Privacy Policy and User Agreement for details. Protein micro array 1. •They allow simultaneous determination of a great variety of analytes from small amounts of samples within a single experiment. Glycans are key players in many biological processes. RNAprotect™ (Ambion) and RNAlater™ (Qiagen) are commercially available solutions that perfuse cells, and ‘freeze’ the cellular RNA profile. See our Privacy Policy and User Agreement for details. Microarray technology for genomic and proteomic research. If you continue browsing the site, you agree to the use of cookies on this website. Important for toxicogenomic studies. (2007). Earlier robots use solid pins, which can only print one feature before needing to collect more DNA from the well. The more pins, the greater the throughput of the robot, but the greater the propensity for pin-to-pin variability. They can be used for studying … Now customize the name of a clipboard to store your clips. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Light emitted from the excited fluorophore travels back through the excitation lens and is focused on the PMT detector. Microarrays for gene expression have made a profound impact in the pharmaceutical and biomedical worlds. The spots are printed on to the glass slide by a robot or are synthesised by the process of photolithography. Microarray technologies allow monitoring of global changes of genes, mRNA and proteins. View abstract . Information from newer microarray technologies such as CGH, ChIP-on-chip, splice variants and microRNAs, combined with gene expression data, can be applied to the drug discovery process enabling many exciting applications. Other researchers use 5% phenol in ethanol which achieves the same result, prior to RNA extraction and purification. role of nuclic acid microarray and protein microarray for drug discovery process 1.introduction about microarray technique and genomics 2.process of drug discovery 3.microarray techiques 4.microarray analysis in drug discovery 5.steps involved in the micro array analysis. Protein microarrays are mainly applied to protein function studies, screening the production of antibodies and recombinant proteins , discovery of proteins implicated in disease or those that are potential drug targets, rapid detection or diagnosis of disease (18,19) screening for protein–protein, DNA–protein and enzyme–substrate interactions (20,21). 4.Toxicological research A rapid platform for the research of the impact of toxin on the cells and their passing on to the progeny. Efficacy or potency, metabolic stability (half-life), and oral bioavailability are also improved in this step of the drug development process. Looks like you’ve clipped this slide to already. Taken together, these studies revealed that interesting proteins identified by proteomics in the intracellular pathways targeted by anticancer drugs can be classified into several categories, including proteins related to mitochondrial function, endoplasmic reticulum (ER) stress proteins, oxidative stress and heat shock proteins, and nuclear proteins involved in signaling transduction. Now customize the name of a clipboard to store your clips. Drug discovery is a multi-process that involves genomics, proteomics, and bioinformatics studies, which lead to the discovery of a new drug entity with a novel mechanism of action. chapter 5 | 26 pages Fabrication of Sol-Gel- Derived Protein Microarrays … See our User Agreement and Privacy Policy. Microbial deterioration of textiles and paper, Customer Code: Creating a Company Customers Love, No public clipboards found for this slide. See our User Agreement and Privacy Policy. The physiological state of a cell is defined by external and internal parameters. •A protein microarray (protein chip)is a high throughput method used to track the interactions & activities of proteins,& to determine their functions,& determining function on a large scale. Probe labelling can be achieved either by direct incorporation of Cy3 or Cy5 UTP into the reverse transcription reaction or by incorporation of UTP containing an allyl amino group followed by subsequent coupling of Cy dye. The drug discovery process was beginning in 19 th century by John Langley in 1905 when he proposed the theory of respective substances. A DNA Microarray is a test that allows for the comparison of thousands of genes at once. microarra y as a gene expression profiling tool microarra y as a comparativ e genomics tool disease diagnosis drug discovery toxicologic al research 22. Abstract: Protein microarray technology is one of the most powerful tools presently available for proteomic studies. If you continue browsing the site, you agree to the use of cookies on this website. If you continue browsing the site, you agree to the use of cookies on this website. Protein Expression for MicroArrays, H.H. Genetic aging Drugs Environment Cell Figure 1. A single-wavelength laser beam scanned back and forth across the array to excite an area representing a single pixel. Proteomic approach of drug discovery includes finding an unstable protein that is causing an undesirable Today drug discovery involves screening hits, medicinal chemistry, and optimization of hits to reduce potential drug side effects (increasing affinity and selectivity). Principles of Clinical Pharmacology NIH, April 26, 2007 Role of FDA in Guiding Drug Development Carl Peck Center for Drug Development Science UCSF, UC-Washington Center – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 52a023-MjJmM Yim, T.G. Haplotype: a set of genetic determinants located on a single chromosome. DOI link for Functional Protein Microarrays in Drug Discovery. Department of Chemistry and Chemical Biology, and Bauer Center for Genomics Research, ... it is discovery-oriented because unknown proteins, as well as known proteins, Protein microarray (protein arrays or protein biochips) technology is a process of immobilizing numerous proteins on to a solid support and using miniaturized assays to describe protein interactions, that has been developed steadily in recent years. INTRODUCTION • Protein micro array(or Protein chip) is a high- throughput method used to track the interactions & activities of proteins, to determinate their functions. Sodium chloride and sodium citrate (SSC) as a source of monovalent cations, these interact mainly with the phosphate groups of the nucleic acids decreasing the electrostatic interactions between the two strands. Protein microarrays for drug candidate profiling. PROTEIN MICROARRAY K.KRUPA SAGAR M.Pharm 1st year 2. (b) The pins are held in a cassette in a rectangular grid, which in turn is held on a robot arm that can be moved between the microtiter well plates and the glass arrays to deposit liquid. Cell communication and signaling ,cell cycle, No public clipboards found for this slide, Role of nuclicacid microarray &protein micro array for drug discovery process. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Thus, being at the heart of medically relevant biological processes, glycans have come onto the scene and are considered hot sp An example of a spotting robot. Looks like you’ve clipped this slide to already. Each pin will spot a different grid on the array (Chapter 4). Expert Opinion on Drug Discovery: Vol. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. You can change your ad preferences anytime. The principle aim of using microarray technology as a gene expression profiling tool is to answer some of the fundamental questions in biology such as "when, where, and to what magnitude genes of interest are expressed. Help to produce effective drugs 20. If you continue browsing the site, you agree to the use of cookies on this website. DNA Microarrays are one of the most effective invention ever developed. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Functional Protein Microarrays in Drug Discovery. 2, No. Microarrays play an increasingly significant role in drug discovery. abnormal proteins may appear which will ultimately affect the other functions ... pharmacogenomics can play a major role in the treatment of patient, with minimised side effects. They are essential for protein folding and stability and act as recognition elements in cell–cell and cell–matrix interactions. Numerous types of protein microarrays have been widely and successfully applied for both basic biological studies and clinical researches, including those designed to characterize protein-protein, protein-nucleic acid, protein-drug/small molecule and antibody-antigen interactions. (c) The number of pins in the cassette can vary. drug targets, design more effective drugs, assessment of drug efficacy and patient response, i.e., to interfere with almost every steps in modern drug discovery process (Ahn et al., 2008). Here, I discuss the role of microarray technology in this burgeoning area. 11, pp. Because of the instability of RNA in general, and the short half-life of most bacterial RNAs in particular, of equal importance to the choice of RNA extraction methodology is how to ‘freeze’ (prevent further production, and degradation of) the RNA in the cell, prior to the extraction procedure. Attractive research tools because they enable researchers to study multiple protein interaction events and activity networks simultaneously. Although nucleic acids may also be considered, their use as drug targets in drug discovery and structure based drug design has been limited due to various effects like toxicity, difficulty in achieving high specificity, etc. The technique has immense potential and promises to play a key role in furthering research in a number of fields, as discussed in this chapter. Harwood Emerging Trend: Cell-Free Protein Expression, F. Katzen and W. Kudlicki Fabrication of Functional Protein Microarrays The Critical Role of Surface Chemistry in Protein Microarrays, A. Guo and X.-Y. An update on using protein microarrays in drug discovery. Clipping is a handy way to collect important slides you want to go back to later. You can change your ad preferences anytime. (d) Most pins in modern use have a reservoir that holds sample and so can print multiple features – usually on different arrays – from a single visit to the well containing probe. Recent advances in proteomic technologies, including both mass spectrometry and protein microarrays, have enabled large-scale screening of proteins in tissues and serum from patients that are applicable for biomarker discovery. 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